alexa 546 conjugated anti cd8 Search Results


cd4 antibody, anti-mouse, apc  (Miltenyi Biotec)
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Miltenyi Biotec cd4 antibody, anti-mouse, apc
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cd11c antibody (ap-mab0814) - azide and bsa free  (Bio-Techne corporation)
90
Bio-Techne corporation cd11c antibody (ap-mab0814) - azide and bsa free
Cd11c Antibody (Ap Mab0814) Azide And Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa 546 conjugated anti cd8  (Vector Laboratories)
86
Vector Laboratories alexa 546 conjugated anti cd8
Alexa 546 Conjugated Anti Cd8, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa 647–labeled anti-b220 mab  (BMA Biomedicals)
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BMA Biomedicals alexa 647–labeled anti-b220 mab
Alexa 647–Labeled Anti B220 Mab, supplied by BMA Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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te2000 fluorescence microscope  (Nikon)
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Nikon te2000 fluorescence microscope
Te2000 Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluor 546 conjugate  (Thermo Fisher)
86
Thermo Fisher alexa fluor 546 conjugate
Alexa Fluor 546 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti-rat alexa fluor 546  (Thermo Fisher)
90
Thermo Fisher goat anti-rat alexa fluor 546
Goat Anti Rat Alexa Fluor 546, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluor 546-tagged anti-human cd8  (Nikon)
90
Nikon alexa fluor 546-tagged anti-human cd8
Cytotoxic activity of human allospecific effector <t>CD8+</t> T lymphocytes. The killing of specific target (human JY B cell line) and irrelevant cell lines (Raji and Daudi) were studied. Cytotoxicity was evaluated by DiOC18(3) – propidium iodide two-color flow cytometric measurement. DiOC18(3)-labeled target cells (10,000 cells per well) were incubated for 3.5 h in the presence of the indicated amounts of effector cells (E/T ratio). For the last 30 min, propidium iodide was added, and the percentage of the live target cells was measured. Specific lysis was calculated as described in Materials and Methods. Results shown represent four independent experiments. (Inset) The result of flow cytometric analysis of CD4/CD8 double-stained forward and side light scatter-gated lymphocytes.
Alexa Fluor 546 Tagged Anti Human Cd8, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd8  (Becton Dickinson)
90
Becton Dickinson cd8
Cytotoxic activity of human allospecific effector <t>CD8+</t> T lymphocytes. The killing of specific target (human JY B cell line) and irrelevant cell lines (Raji and Daudi) were studied. Cytotoxicity was evaluated by DiOC18(3) – propidium iodide two-color flow cytometric measurement. DiOC18(3)-labeled target cells (10,000 cells per well) were incubated for 3.5 h in the presence of the indicated amounts of effector cells (E/T ratio). For the last 30 min, propidium iodide was added, and the percentage of the live target cells was measured. Specific lysis was calculated as described in Materials and Methods. Results shown represent four independent experiments. (Inset) The result of flow cytometric analysis of CD4/CD8 double-stained forward and side light scatter-gated lymphocytes.
Cd8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti–metallophilic macrophage ab (moma-1  (BMA Biomedicals)
90
BMA Biomedicals rat anti–metallophilic macrophage ab (moma-1
Cytotoxic activity of human allospecific effector <t>CD8+</t> T lymphocytes. The killing of specific target (human JY B cell line) and irrelevant cell lines (Raji and Daudi) were studied. Cytotoxicity was evaluated by DiOC18(3) – propidium iodide two-color flow cytometric measurement. DiOC18(3)-labeled target cells (10,000 cells per well) were incubated for 3.5 h in the presence of the indicated amounts of effector cells (E/T ratio). For the last 30 min, propidium iodide was added, and the percentage of the live target cells was measured. Specific lysis was calculated as described in Materials and Methods. Results shown represent four independent experiments. (Inset) The result of flow cytometric analysis of CD4/CD8 double-stained forward and side light scatter-gated lymphocytes.
Rat Anti–Metallophilic Macrophage Ab (Moma 1, supplied by BMA Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa 546–labeled pna  (BMA Biomedicals)
90
BMA Biomedicals alexa 546–labeled pna
Cytotoxic activity of human allospecific effector <t>CD8+</t> T lymphocytes. The killing of specific target (human JY B cell line) and irrelevant cell lines (Raji and Daudi) were studied. Cytotoxicity was evaluated by DiOC18(3) – propidium iodide two-color flow cytometric measurement. DiOC18(3)-labeled target cells (10,000 cells per well) were incubated for 3.5 h in the presence of the indicated amounts of effector cells (E/T ratio). For the last 30 min, propidium iodide was added, and the percentage of the live target cells was measured. Specific lysis was calculated as described in Materials and Methods. Results shown represent four independent experiments. (Inset) The result of flow cytometric analysis of CD4/CD8 double-stained forward and side light scatter-gated lymphocytes.
Alexa 546–Labeled Pna, supplied by BMA Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti-cd8 percp-cy5.5  (Thermo Fisher)
90
Thermo Fisher rat anti-cd8 percp-cy5.5
Cytotoxic activity of human allospecific effector <t>CD8+</t> T lymphocytes. The killing of specific target (human JY B cell line) and irrelevant cell lines (Raji and Daudi) were studied. Cytotoxicity was evaluated by DiOC18(3) – propidium iodide two-color flow cytometric measurement. DiOC18(3)-labeled target cells (10,000 cells per well) were incubated for 3.5 h in the presence of the indicated amounts of effector cells (E/T ratio). For the last 30 min, propidium iodide was added, and the percentage of the live target cells was measured. Specific lysis was calculated as described in Materials and Methods. Results shown represent four independent experiments. (Inset) The result of flow cytometric analysis of CD4/CD8 double-stained forward and side light scatter-gated lymphocytes.
Rat Anti Cd8 Percp Cy5.5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


Cytotoxic activity of human allospecific effector CD8+ T lymphocytes. The killing of specific target (human JY B cell line) and irrelevant cell lines (Raji and Daudi) were studied. Cytotoxicity was evaluated by DiOC18(3) – propidium iodide two-color flow cytometric measurement. DiOC18(3)-labeled target cells (10,000 cells per well) were incubated for 3.5 h in the presence of the indicated amounts of effector cells (E/T ratio). For the last 30 min, propidium iodide was added, and the percentage of the live target cells was measured. Specific lysis was calculated as described in Materials and Methods. Results shown represent four independent experiments. (Inset) The result of flow cytometric analysis of CD4/CD8 double-stained forward and side light scatter-gated lymphocytes.

Journal:

Article Title: Kv1.3 potassium channels are localized in the immunological synapse formed between cytotoxic and target cells

doi: 10.1073/pnas.0307421100

Figure Lengend Snippet: Cytotoxic activity of human allospecific effector CD8+ T lymphocytes. The killing of specific target (human JY B cell line) and irrelevant cell lines (Raji and Daudi) were studied. Cytotoxicity was evaluated by DiOC18(3) – propidium iodide two-color flow cytometric measurement. DiOC18(3)-labeled target cells (10,000 cells per well) were incubated for 3.5 h in the presence of the indicated amounts of effector cells (E/T ratio). For the last 30 min, propidium iodide was added, and the percentage of the live target cells was measured. Specific lysis was calculated as described in Materials and Methods. Results shown represent four independent experiments. (Inset) The result of flow cytometric analysis of CD4/CD8 double-stained forward and side light scatter-gated lymphocytes.

Article Snippet: Cells were labeled with Alexa Fluor 546-tagged anti-human CD8 and CD8 + cells were identified for current recording by using a Nikon TE2000 fluorescence microscope.

Techniques: Activity Assay, Labeling, Incubation, Lysis, Staining

Enrichment of Kv1.3/FLAG in or around the IS. Distribution of Kv1.3/FLAG (red: anti-FLAG followed by Alexa Fluor 546-RAMIG), MHC class I (green: X-FITC-W6/32), and CD8 (Alexa Fluor 647-anti-CD8) molecules in a lone CTL (Top) and CTL-JY target cell conjugates (Middle and Bottom). The thickness of the optical slice was 1.1 μm. In lone CTLs, the channels are distributed in small patches throughout the membrane (Top). On CTL-target interaction Kv1.3 channels are redistributed and enriched in the IS in the majority of CTLs (Middle) or surround the synapse like a ring (Bottom). (Scale bar, 5 μm.)

Journal:

Article Title: Kv1.3 potassium channels are localized in the immunological synapse formed between cytotoxic and target cells

doi: 10.1073/pnas.0307421100

Figure Lengend Snippet: Enrichment of Kv1.3/FLAG in or around the IS. Distribution of Kv1.3/FLAG (red: anti-FLAG followed by Alexa Fluor 546-RAMIG), MHC class I (green: X-FITC-W6/32), and CD8 (Alexa Fluor 647-anti-CD8) molecules in a lone CTL (Top) and CTL-JY target cell conjugates (Middle and Bottom). The thickness of the optical slice was 1.1 μm. In lone CTLs, the channels are distributed in small patches throughout the membrane (Top). On CTL-target interaction Kv1.3 channels are redistributed and enriched in the IS in the majority of CTLs (Middle) or surround the synapse like a ring (Bottom). (Scale bar, 5 μm.)

Article Snippet: Cells were labeled with Alexa Fluor 546-tagged anti-human CD8 and CD8 + cells were identified for current recording by using a Nikon TE2000 fluorescence microscope.

Techniques: Membrane